產(chǎn)品英文名稱:WNT-3a, Human 產(chǎn)品中文名稱:人重組WNT-3a蛋白 人重組WNT-3a蛋白 產(chǎn)品簡介: StemRD已研發(fā)出一種新穎的表達(dá)和純化諸如WNT蛋白等“困難”蛋白的*方法。我們*的處理方法能得到純度和活性均于市場方法生產(chǎn)的WNT蛋白。StemRD的WNT蛋白絕不含有現(xiàn)今市場普遍使用的WNT蛋白中含有的某些非特異性成分。 WNT-3a 是WNT 信號蛋白家庭的成員之一,在胚胎成長和成體組織維護(hù)方面起關(guān)鍵作用。WNT-3a 是典型的通過β-聯(lián)蛋白通路發(fā)送信號。已有規(guī)格的WNT-3a是一個(gè)包含328個(gè)氨基酸殘基的單鏈蛋白質(zhì)。由于糖基化,WNT-3a 在非還原條件下用SDS-PAGE處理會明顯損失掉38-41 kDa分子量。StemRD公司生產(chǎn)的WNT-3a是用人體細(xì)胞系中Wnt-3a cDNA 在無蛋白培養(yǎng)基上過度表達(dá)得到。使用*的純化方法,區(qū)別于其它已發(fā)表的純化方法,提高蛋白純度。 人重組WNT-3a蛋白 產(chǎn)品特性: » WNT-3a使用LC/MS 層析,純度大于98%,結(jié)構(gòu)經(jīng)過NMR驗(yàn)證 » 生物活性認(rèn)證:經(jīng)過生物試驗(yàn)驗(yàn)證,優(yōu)于其它同類型產(chǎn)品 » WNT-3a 是典型的通過β-聯(lián)蛋白通路發(fā)送信號 » 僅限用于科學(xué)研究,非人體使用 WNT-3a, Human 產(chǎn)品英文簡述: We express and purify WNT proteins by using a proprietary process that is distinctive from the published protocol. Our method results in WNT proteins that are purer and more active than market-leading products which have been associated with non-specific artifacts. WNT-3a is a member of the WNT family of signaling proteins that play key roles in embryonic development and the maintenance of adult tissues. WNT-3a is a prototypic canonical WNT that signals through the β-catenin pathway. The predicted size of human WNT-3a is a monomeric protein containing 328 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of 38 - 41 kDa on SDS-PAGE under non-reducing conditions. StemRD’s WNT-3a is produced from a human cell line overexpressing human Wnt-3a cDNA in protein-free medium. Purification is done by a proprietary process that is distinct from the published method. WNT all compounds are: 1. >98% pure by LC/MS analysis, and structurally verified by NMR. 2. Bioactivity Certified: tested for biological activities, an advent-age over other supplies. 重慶市華雅干細(xì)胞技術(shù)有限公司 |